Fig. 3. (A-L) Analysis of the patterns of embryonic RA response during maternal RA supplementation. Embryos carrying the RARE-hsp68-lacZ RA reporter transgene, collected from RA-supplemented or untreated mothers, were concomitantly X-gal stained. (A-D) Profile views of E9.5 embryos. (E-I) Dorsal views of the forelimb bud region of E9.5 embryos. Brackets indicate the outgrowing forelimb buds. (J,K) Dorsal views of the forelimbs of E10.5 embryos. (L) Transverse section of an E10.5 embryo at the level of the hindlimb buds. WT, wild-type embryos; –/–, Raldh2^–/– embryos; –RA, untreated embryos. The embryos in B-D and F-I were RA-treated from E7.5 to E8.5 with 100 µg/g food and from E8.5-E9.5 with 100 µg/g food or 250 µg/g food, as indicated in the panels (100 and 250, respectively). The embryo in K was RA-treated until E10.5, and the one in L until E9.5 (250 µg/g food). (M-P) Detection of RARß transcripts in E9.5 wild-type (M,N) and Raldh2^–/– (O,P) embryos that were untreated (M,P) or RA-treated from E7.5 to E9.5 (250 µg/g food) (N,O). Arrowheads point to the similar rostral expression boundaries in the hindbrain of untreated and RA-treated wild-type embryos. Profile views. (Q,R) RARß transcript distribution in the forelimbs of E10.5 wild-type (Q) and Raldh2^–/– (R) embryos that received RA from E7.5 to E10.5 (250 µg/g food). Dorsal views. fl, forelimb buds; hl, hindlimb buds; mn, mesonephros; sc, spinal cord; so, somite.

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