Fig. 6. Expression of dHand in Raldh2^–/– forelimbs. (A-C) Wild-type (A) and Raldh2^–/– (B,C) forelimb buds analyzed at E10.5 after short-term and long-term RA supplementation, respectively. Note the lack of posterior asymmetry of dHand expression in mutant forelimbs. (D-G) Early expression pattern of dHand in wild-type (D) and Raldh2^–/– (E-G) forelimb buds analyzed at E9-9.5 after short-term (D,E,G) and long-term (F) RA supplementation. Although dHand transcripts are posteriorly restricted during early forelimb outgrowth in mutants (brackets), only the anteroproximal forelimb margin remains free of dHand transcripts during further limb growth (arrowheads).

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