Fig. 1. Dorsal sensory neurons develop within the Dpp signaling region in the dorsal ectoderm. (A,B) Merged confocal images of PNCs (cell clusters) and SOPs (single cells) visualized by immunostaining using anti-Ato (green in A) or anti-Ac (green in B) antibodies in two consecutive abdominal segments of staged 11 embryos. Anterior is towards the left and dorsal is upwards. The Dpp signal receiving cells in a dad-lacZ enhancer trap line are visualized by anti-ß-galactosidase antibodies (red). Orthogonal sections are indicated by the blue lines (I and II in A, and III and IV in B) and shown in A', A'' and B', B'', respectively. Merged images are shown in color; black and white images represent the separate red (middle) and green (right) channels. In A, the C1 SOP, which contributes to one of the chordotonal organs, is marked by a stippled line (A') and originates within the dad-lacZ domain (red). The SOP giving rise to the dorso-bipolar neuron (dbp) is in the process of delamination (A'') and also originates within the dad-lacZ domain. The more ventrally located PNC cluster, from which the C2-C5 chordotonal SOPs will be singled out, is only partially within the dad-lacZ domain. In B, the Ac-positive PNCs 1 and 3 originate within and outside the dad-lacZ domain, respectively (B'). The delaminating SOPs 2 and 4 form within the dad-lacZ domain (B',B'').

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