Fig. 5. elB mutant tracheal phenotypes. (A) Lumen staining of wild-type embryo at stage 14. (B) In elB⁴⁷ mutant embryos, the lateral trunk anterior branch is not detected (arrow), and fusion between lateral trunks in adjacent metameres is not observed. (C) Anti-Trh staining of a wild-type embryo at stage 12 shows the initial stages of branching. (D) In elB mutants at the same stage, the lateral trunk anterior branch is stalled. (E) Wild-type embryo at stage 14. (F) In elB mutants, the lack of lateral trunk anterior migration is evident by stage 14. (G,I) Lumen and anti-Trh staining of the dorsal branches of a wild-type embryo. (H,J) In elB mutants, fusion between adjacent dorsal branches is observed (arrows). (K,L) Rescue of the tracheal phenotype of elB⁴⁷ mutant embryos by btl-Gal4/UAS-elB was examined. Embryos misexpressing ElB were identified by anti-ElB staining, and stalled visceral branch migration (upper arrowhead in K). Over 120 such embryos were examined. elB⁴⁷ embryos were scored by subtle defects in dorsal and ganglionic branch migration (arrowheads). Complete rescue of LTa migration (arrow in K) and partial rescue of dorsal branch migration (arrow in L) were observed.

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