Fig. 6. Effects of soluble ephrin-B1-Fc on neural crest cell migration. Pre-clustered Fc or ephrin-B1-Fc (10 µg/ml) was added to the bottom well of a chemotaxis chamber and neural crest cells were added to the top well. In some control experiments preclustered ephrin-B1-Fc was also added to the upper well. Cells that migrated to the bottom side of the porous filter were stained, solubilised and quantified by OD measurements. Ephrin-B1-Fc induces a significant decrease of early migratory neural crest cell migration (white bars). In contrast, clustered ephrin-B1-Fc causes a significant increase in the transfilter migration of melanoblast cells (grey bars). This is a representative experiment and each data point is the mean ± s.e.m. of triplicate wells (P<0.05).

Return to article