Fig. 2. Immunocytochemical detection of MSY4 and MSY4-HA. (A,C,E) Testis sections from PMP transgenic mice. (B,D,F) Testis sections from PMH transgenic mice. (A,C) Serial sections of a stage VI tubule from a PMP animal. (B,D) Serial sections of a stage VI tubule from a PMH animal. (A,B) Endogenous MSY4 detected with MSY4 antibody in pachytene spermatocytes (ps) and round spermatids (rs). The transgenic MSY4-HA proteins lack the N-terminal epitope recognized by the MSY4 antibody. (C,D) MSY4-HA detected with HA antibody in elongated spermatids (eds) of PMP and PMH animals, respectively. (E) Detection of MSY4-HA expression in elongated spermatids in a stage VII tubule of a PMP transgenic testis. Notice the absence of MSY4-HA in round spermatids. (F) Expression of MSY4-HA in round and elongated spermatids of a stage VII tubule from a PMH testis. Sections were counterstained with Hematoxylin.

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