Fig. 6. Analysis of total testis basic proteins from sonication sensitive (SS) and sonication resistant (SR) spermatid nuclei from wild-type and transgenic animals. Total testis basic protein preparations were fractionated by urea-gel PAGE and detected with napthol blue-black staining. Though the total yield of protein was reduced in PMH and PMP basic protein preparations, an equivalent amount of protein was loaded in each lane (15 µg). The left-hand panel contains samples prepared from testis tissue, while the right-hand samples were prepared from epididymal tissue. Wild-type controls are shown in lanes 2, 5, 8 and 11. Lanes 1, 4, 7 and 10 contain protein isolated from PMH mice, while lanes 3, 6, 9 and 12 contain protein isolated from PMP mice. The positions of transition protein 2 (TP2), protamine 1 (PRM1) and mature protamine 2 (PRM2) are labeled. The PRM2 precursor, the partially processed forms of PRM2 and an abnormally processed form of PRM2 are all labeled by PRM2*.

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