Fig. 7. Electrophoretic mobility shift assay analysis of wild-type and transgenic testis extracts. Lanes 1-3, wild-type testis extracts; lanes 4-9, PMH extracts; and lanes 10-15, PMP extracts. RNA containing the wild-type YRS (wt YRS) was used in lanes 1-8 and lanes 10-14. RNA containing a YRS mutation (C26A) that disrupts MSY4 binding (Giorgini et al., 2001) was used in lanes (9) and (15). Supershifts of the EMSA complex were carried out using either MSY4 antibody (lanes 2, 6, 12), HA antibody (lanes 3, 7, 13) or both (lanes 8 and 14). As a control, pre-immune sera was added to reactions in lanes 5 and 11. No sera was added to reactions in lanes 1, 4 and 10.

Return to article