Fig. 4. Early expression of Hro-eve provides no evidence for pair rule patterning. (A) Bright-field (Nomarski optics) image of a stage 7 embryo processed by in situ hybridization for Hro-eve. Staining is uniform within and among bandlets, except for punctate staining associated with apparent mitotic figures in three teloblasts; arrow indicates prophase; yoked arrows indicate telophase (left) and anaphase (right). An enlarged view (B) at a slightly different focal plane of the outlined region of the same embryo shows perinuclear staining of Hro-eve in blast cells of the bandlets (arrowheads), but no alternating intensity of staining that would be indicative of a pair-rule expression pattern. Scale bar: 100 µm in A; 30 µm in B.

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