Fig. 7. Ganglionic cells expressing Hro-eve arise from the N lineage. Pseudo-colored confocal images of sectioned embryos that had been fixed and stained for Hro-eve transcripts (green) at stage 9 and in which 1 or more cells had been injected with RDA (red) at stage 6a. (A) A horizontal view of approx. four segmental ganglia in which the O, P and Q lineages are labeled on the left and the N lineage is labeled on the right. Anterior is upwards; the plane of section is oblique. In each hemiganglion, the posterior spot of Hro-eve expression lies just posterior to the main lobe of OPQ-derived cells (arrows) and in the anterior edge of the posterior half-ganglion (yoked arrows). The anterior spots of Hro-eve expression (yoked arrowheads) lie at the anterior edge of the ganglion. (B,C) Transverse sections through the ventral nerve cord of an embryo in which an N teloblast had been labeled; Hro-eve-positive neurons in the anterior spot (B, arrowheads) and posterior spot (C, arrows) colocalize with N-derived cells. Yolk platelets (y) exhibit background fluorescence in the RDA channel; background in situ signal is present between yolk platelets and imperfections in the sectioned material (*) also appear green in the pseudo-colored images. Scale bar: 50 µm in A; 30 µm in B,C.

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