Fig. 1. osk mRNA maintenance at the posterior pole of the oocyte requires Long Osk. (A) osk mRNA in situ hybridization to whole-mount ovaries (left panels) and freshly laid eggs (right panels; anterior is towards the left and dorsal towards the top) of osk RNA-null females osk^A87/Df(3R)p-XT103, expressing either a wild-type osk transgene (upper panels), oskM1R encoding Short Osk (middle panels) or oskM139L encoding Long Osk (lower panels). The arrow indicates residual osk mRNA in embryos produced by females expressing Short Osk alone. (B) Comparison of the delocalization pattern of osk mRNA in stage 10 oocytes of the osk nonsense mutant osk⁸⁴/Df(3R)p-XT10 (upper panel) and of the RNA-null mutant osk^A87/Df(3R)p-XT103 expressing oskM1R. (C) Western blot analysis of Osk isoforms produced in ovaries shown in A, and of -Tubulin (an internal loading control). Oregon R is the wild-type reference strain.

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