Fig. 4. Proper patterning requires the combinatorial activity of Long Osk and Short Osk. (A) Hatching rate of embryos and cuticle analysis of unhatchers with patterning defects produced by osk RNA-null females osk^A87/Df(3R)p-XT103, expressing the indicated transgenes. Number of eggs scored to calculate hatch rates are osk wild type (n=90); oskM1R [n=125 (line 1) and 49 (line 2)]; oskM1R+oskM139L [n=561 (line 1) and 147 (line 2)]; oskM139L (n>300). [(1) and (2) refer to the lines analyzed by western blotting in Fig. 3E.] *The remaining 12% of unhatchers develop cuticles without obvious patterning defects. (B) Posterior defects (left panel), anterior defects (middle panel), and both anterior and posterior defects (right panel) observed in progeny of females expressing only oskM1R. None of these defects is detected when oskM1R and oskM139L are co-expressed (C). Anterior is towards the top and ventral is towards the left.

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