Fig. 5. Long and Short Osk are required for efficient pole cell formation. (A-C) Vasa immunostaining reveals pole cells in embryos produced by osk^A87/Df(3R)p-XT103 females expressing a wild-type osk transgene (A), the two transgenes oskM1R and oskM139L (B) or only oskM1R (C). Compared with embryos produced by females expressing both Osk isoforms (A,B), a significant reduction in pole cell number is observed in embryos produced by females expressing only Short Osk (C). (D) Fertility of the adult female progeny was evaluated by ovary dissection. Note that the two independent lines expressing oskM1R or both oskM1R and oskM139L were subjected to Osk western blotting analysis [denoted as (1) and (2) in Fig. 3E]. The number of ovaries dissected in each case was oskWT (n=41), oskM1R [n=54 (line 1) and 51 (line 2)], oskM1R and oskM139L [n=52 (1) and 61 (2)].

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