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Fig. 7. The N-terminal extension of Long Osk is not sufficient for posterior anchoring. ß-Galactosidase staining of wild-type (A,B) and osk84/Df(3R)pXT103 ovaries (C,D) expressing the m1414lacwt transgene, which encodes a translational fusion of the first 139 amino acids of Long Osk to ß-galactosidase (Gunkel et al., 1998). The chimeric protein is detected at the posterior pole of wild-type oocytes during stage 10 onwards (A,B), whereas in the absence of endogenous Osk activity, the fusion delocalizes during stage 10 (C,D).