Fig. 1. Analyses of Cctra transcripts. (A) Northern blot analysis on poly A+ RNA from embryos (E), larvae (L), and adult males (M) and females (F), using as probe the F1 cDNA clone. In males, two predominant transcripts 1.9 kb and 2 kb long are detected, while two different transcripts 1.6 kb and 3 kb long are present in females. In embryos, two transcripts are detected and in larvae all four transcripts are detected. (B) RT-PCR amplification of Cctra on adult males (M) and females (F) total mRNA samples. Three main products are present in the female lane (F), which are 0.7 kb, 1.3 kb and 2.1 kb long. In the male lane (M) four bands are detectable which are 1 kb, 1.1 kb, 1.3 kb and 2.1 kb long. Male (cM) and female (cF) RT-PCR negative controls (reactions without reverse transcriptase) are shown.

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