Fig. 4. Phenotypic analysis of RNAi intersexes. (A) Wild-type female has long pigmented bristles on the femur pointing towards the coxa of the foreleg (arrow in E) and the ovopositor (A,I). (B) Wild-type male exhibits two spatulated bristles on the head (B), a row of non-pigmented bristles on the ventral part of the femur towards the coxa of the foreleg, short pigmented bristles grouped on the dorsal part of the femur (arrow in F) close to the coxa of the foreleg (F) and male genitalia (B,O). (C,D) Intersexes obtained by dsRNA injection into the anterior pole of the embryos exhibit male-specific spatulated bristles on the head (arrow in C), male-specific bristles (upper arrow in H) and female-specific bristles (arrow in G; lower arrow in H) mixed together on the femur of the foreleg (G,H) and female genitalia (C,D). Some intersexes show various degrees of abnormal gonadal development exhibiting bent (arrow in D), deformed (L-N) or completely absent (arrow in P) genitalia. Scale bar in D applies at A-D; scale bar in H applies to E-H; scale bar in I applies to I; scale bar in L applies to L; and scale bar in P applies to M-P.

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