Fig. 5. Dark-field images of in situ hybridization demonstrating trkC_Tr mRNA in developing vibrissa follicles of E14.5 mice. (A,B) In normal fetuses, extensive labeling (between arrowheads) is present in follicles with the probe recognizing all isoforms of trkC (B), but virtually no labeling is present with the probe that recognizes only full length kinase forms of trkC (A). (C) In trkC_K^–/– animals, labeling within the follicle is still present (between arrowheads) with the probe that recognizes all forms of trkC indicating that this mutation does not eliminate the expression of truncated trkC within the follicle. (D) Virtually no labeling occurs in the follicles of trkC_E^–/– mutants with the probe for all isoforms of trkC, indicating that expression of trkC_Tr within follicles was eliminated. Scale bar: 50 µm.

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