Fig. 3. Rho1 accumulates at sites of DE-cadherin localization in the embryo and ovary. (A-C) Confocal image of a hindgut tube from a stage 14 embryo showing double labeling for both DE-cadherin (A) and Rho1 (B). A higher magnification view of the merged image is shown in C. In all merged images, Rho1 staining is red and the staining for other molecules is green. Apical is up in all images. Note the apical accumulation of Rho1 protein that coincides with DE-cadherin expression (arrow in C). (D-F) A stage 14 hindgut tube double labeled for the septate junction protein neurexin (D), Rho1 (E), and the merged image (F). Note that the neurexin staining (green) is largely exclusive of Rho1 apical accumulation (red). (G-I) Control staining documenting DE-cadherin (green) localization relative to neurexin (red). (J) A schematic diagram of the gut showing the relative locations of the adherens junctions (AJ) and septate junctions (SJ). (K-M',T-U) Expression of Rho1 protein during oogenesis. Rho1 protein is expressed in all stages, including the germarium (K). Note Rho1 accumulation in the apical regions of follicle cells (L',M'; arrows in L,M). Rho1 expression is upregulated in border cells (T') and accumulates at lateral follicle cell contacts (cross section, T''; grazing section, U) and in the cortex of the oocyte (arrows in T,T''). (N-P',V-W) Localization of DE-cadherin during oogenesis. Cadherin is upregulated in the border cells (P', arrowhead in V;V') and also localized to lateral follicle cell contacts (arrow in V; V'', W). (Q-S',X-Y) Merged images of Rho1 and DE-cadherin staining (red: Rho1, green: DE-cadherin). Stages of oogenesis shown are the germarium (K,N,Q), stage 3 and 5 (L,O,R), stage 8 (M,P,S) and stage 10a (T,V,X). Scale bars: A,D,G: 0.6 µm; C,F,I: 0.2 µm; Q,R,S,X: 30 µm; X',X'',Y: 10 µm.

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