Fig. 4. Embryos mutant for Rho1 show aberrant DE-cadherin and catenin localization. Confocal micrographs showing junction protein expression in wild-type and Rho1 mutant embryos. (A-B,E-F) DE-cadherin expression in stage 14 (A,B) and stage 15 (E,F) wild-type embryos. (C-D,G-H) DE-cadherin expression in stage 14 (C,D) and stage 15 (G,H) Rho1 mutant embryos. Note the disruption of DE-cadherin localization near the leading edge (arrow in H), but not in more lateral regions (arrowhead in H). (I-L) No difference in neurexin expression (septate junctions) is observed in the stage 15 Rho1 mutant embryo (K,L) compared to wild type (I,J). DE-cadherin and neurexin were simultaneously imaged in the same wild-type and Rho1 mutant embryos. Brackets in D indicate the leading edge, and in H,L,P,T, the dorsal midline. (M-P) ß-catenin expression is disrupted in stage 15 Rho1 mutants (O,P) compared to wild-type embryos (M,N). (Q-T) -catenin expression is disrupted in stage 15 Rho1 mutants (S,T) compared to wild-type embryos (Q,R). In all images, anterior is left. Dorsal is up in A-D. E-T are dorsal views. Boxes in A,C,E,G,I,K,M,O,Q,S indicate the region of the embryo shown in B,D,F,H,J,L,N,P,R,T, respectively. Scale bars: A: 50 µm, B: 10 µm.

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