Fig. 6. Rho1 localization is aberrant in catenin mutants. (A-B') Rho1 expression in stage 15 embryos homozygous for a deficiency that removes the p120^ctn locus. (C-F') Rho1 (C-F) and ß-galactosidase (E',F') expression in stage 15 puc-lacZ/TM3 embryos injected with p120^ctn dsRNA. Note the accumulation of Rho1 protein at the leading edge in both deficiency and RNAi embryos (arrows in A',B',E,F), not observed in either ftz RNAi embryos (G-H') or other dorsal closure mutants basket (I,K) and hemipterous (J,L) (arrows in K, L; compare with A',B'). (M-O') Rho1 (M,N,O) and -spectrin (N',O') expression in stage 6 embryos injected with -catenin dsRNA. (P,Q) Rho1 (P) and DE-cadherin (Q) expression in embryos injected with -catenin dsRNA. Note apical localization of DE-cadherin protein in some cells (arrow in Q). In all images, anterior is left. Scale bars: A,C,G,I,M: 50 µm; A',E,H,K,N,P: 25 µm.

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