Fig. 5. The effect of noggin-conditioned medium on lens cell elongation stimulated by vitreous humor or IGF-1. (A) Vitreous humor caused lens epithelial cells to elongate to over 18 µm in 5 hours in the presence of conditioned medium from CHO cells transfected with an empty plasmid (P<<0.001). Medium from CHO cells transfected with a plasmid encoding noggin reduced cell elongation stimulated by vitreous humor to approximately 14 µm (P<0.01). (B) Conditioned medium from cells expressing noggin had no effect on lens epithelial cell elongation stimulated by recombinant chicken IGF-1 (16 ng/ml; P=0.36). Error bars are ± s.e.m.

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