Fig. 6. The elongation-promoting activity removed by noggin was restored by adding BMPs. Vitreous humor was incubated with recombinant Fc-noggin and the noggin-bound material was removed with Protein A/G beads. Protein A/G beads alone did not remove the elongation-promoting activity. Treatment of vitreous humor with Fc-noggin reduced cell elongation to approximately 17 µm from over 20 µm. Addition of BMP-2, -4, or -7 restored full elongation (P<0.02 for each BMP added to noggin-depleted vitreous humor compared to cells cultured in noggin-depleted vitreous humor alone). Error bars are ± s.e.m.

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