Fig. 7. Disruption of the GCL ChAT-positive array within hours of exposure to pharmacological agents affecting MTs. (A) The low doses of drugs used do not significantly alter the retina stratification 4 hours after treatment (normal is on the left; demecolchid treated is on the right). Notice the accumulation of mitotic cells at the top of the demecolchid treated retina (right). (B,C) The regular organization of the GCL ChAT-positive array is altered 2 hours after administration of demecholcid (B) or paclitaxel (C). Scale bars: in A, 20 µm in A; in C, 10 µm in B,C. (D, top) The distribution of Delaunay segments associated with the GCL ChAT-positive array is altered 2 hours after administration of 10 µM demecolchide, and recovers by 24 hours (black symbols are examples of treated cases; grey lines correspond to normal plots). (D, bottom) Examples of the effects of 5 µM paclitaxel 4 and 24 hours after administration (black symbols indicate treated cases, grey line indicates normal cases). The treated cases are statistically different from normal 2 and 4 hours after treatment, but not 24 hours after treatment (KS test, P<0.01 and bootstrap comparison of the datasets).

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