Fig. 4. Insufficiency of dpp expands the Iro-C domain (green). (A) Second instar wild-type wing disc: notum (green, Iro-C), wing pouch (red, Nub protein) (Ng et al., 1995), hinge (unlabelled territory, arrowhead). (B-D) Second, early third and late third instar dpp^d12/dpp^d14 discs stained as in A. Iro-C ectopically accumulates in the hinge territory (arrowhead in B); later it is removed from part of it (arrowheads in C,D). (E,F) Tsh in wild-type and dpp^d12/dpp^d14 late third instar discs, respectively, accumulates most strongly in the hinge territory (arrowheads). (G,H) Sensory organ mother cells (anti-Senseless antibody, red) in wild-type and dpp^d12/dpp^d14 late third instar discs, respectively. Presumptive hinge groups (arrowheads) are shown at higher magnification in insets. (I,J) Tsh (red) and Iro-C in wild-type (I) and dpp^d12/dpp^d14 (J, merged and separate channels) second instar discs. To emphasize detail, discs are reproduced at different magnifications.

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