Fig. 2. Loss of EDTA/EGTA-sensitive adhesion sites in cadA^– cells. Cells were developed in 17 mM phosphate buffer (pH 6.4) for 4 hours. Cell aggregates were dissociated mechanically and cell reassociation was assayed in the presence of 10 mM EDTA or 10 mM EGTA. (A) Reassociation of KAX3 in the presence of EDTA () or EGTA (), or in their absence (). (B) Reassociation of cadA-10 cells in the presence of EDTA () or EGTA (), or in their absence (). (C) Effects of soluble DdCAD-1 and anti-DdCAD-1 Fab on the reassociation of cadA-10 cells. Mutant cells were allowed to reassociate in the absence () or presence ()of 50 µg/ml soluble DdCAD-1 or 50 µg/ml anti-DdCAD-1 Fab () or 20 mM carnitine (). Data represent mean±s.d. (n=3).

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