Fig. 5. Aberrant cell sorting patterns in cadA^– slugs. (A-C) The distribution of prestalk and prespore cell in wild-type and mutant slugs was examined using cells stained with Neutral Red: (A) wild-type slugs; (B,C) cadA^– slugs. (D-H) The prestalk-prespore pattern was examined using GFP-expressing transformants. Strain JS24 (cotB::GFP; cadA⁺) was derived from KAX3 (cadA⁺) and strain TL144 (cotB::GFP; cadA^–) was derived from TL98 (cadA^–). (D) JS24 cells were developed on 2% agar for 20 hours and slugs showed the normal sorting pattern. (E) TL144 cells showed low levels of GFP expression at the mound stage and GFP-labeled cells were present in a nodule-like protrusion (arrowheads): phase micrograph (left panel); epifluorescence micrograph (right panel). (F) TL144 slug showing the non-sorted pattern. (G) TL144 slugs with an abnormally large anterior zone of unlabeled cells. (H) TL144 slugs showing aberrant sorting patterns. Scale bars: 1 mm.

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