Fig. 1. Transplant scheme and DML evaluation. (A) Schematic representation of the tissues used to replace an ablated DML. (B) Confocal image stack of an E2.5 quail embryo immunolabelled with anti-desmin (red) and qcpn (green) antibodies. (C) Schematic of B showing the DML as a cluster of quail epithelial cells at the dorsomedial limit of the somite, which are also lightly labelled by the desmin antibody. (D) Transverse section of an E3 chick somite that received a quail DML transplant 24 hours prior to harvest. Desmin immunoreactivity marks the myotome and some cells within the DML and lateral dermomyotome. The bar indicates the extent of donor-derived myotome as determined by the presence of quail nuclei in an adjacent serial section (shown in Fig. 4). (E) Schematic of D showing the position of the myotome, dermomyotome and DML with areas comprising quail nuclei shaded. Abbreviations: CPM, cranial paraxial mesoderm; dm, dermomyotome; DML, dorsomedial lip of the dermomyotome; LPM, lateral plate mesoderm; M, medial; mt, myotome; NT, neural tube; PSM, presomitic mesoderm; R, rostral; VLL, ventrolateral lip of the dermomyotome. The rostral direction is towards the right in B,C and in subsequent whole-mount images.

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