Fig. 2. Transplant of non-dermomyotome tissues to a DML position. (A-D) Confocal z-series images immunolabelled with anti-desmin and qcpn antibodies to label the myotome (red) and quail (green), respectively. (A,B) Transplant of LPM from the level of the rostral PSM (Table 1, 1.07). (A) The expansion of the transplanted quail tissue is comparable with that of neighbouring myotomes. The quail tissue remained within the rostral and caudal boundaries of the host somite (outlined with a broken line) and expanded dorsomedially, but no epithelial DML structure was apparent. (B) Desmin labelling shows truncation of the myotome in the operated somite from the level of the DML ablation (arrowheads). Note robust growth of the neighbouring myotomes. (C,D) CPM transplant (Table 1, 2.04). (C) The quail tissue expanded dorsomedially in concert with the neighbouring somites and has largely remained within the somite boundaries. Arrows indicate encroachment of quail nuclei in cells that have crossed somitic boundaries. (D) The myotome of the operated somite is truncated in comparison to neighbours corresponding to the site of the DML ablation (arrowheads).

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