Fig. 4. BrdU incorporation into the epaxial myotome (2 hours post administration). (A-C) Transverse cryosections through an embryo that received a DML transplant from ssXIII of a 25 somite quail embryo to ssXI at 26 somites and was harvested the following day, 2 hours after BrdU administration. (A) Section through the axial mid region of the recombinant somite at the plane of the myocyte nuclei reacted to label quail cells. Two layers of quail nuclei can be seen; the medialmost (lower) layer corresponds to the myotome (mt) and the more superficial layer the dermomyotome (dm). (B) The same section as A showing BrdU-labelled cells. (C) A composite of A and B, showing BrdU co-localised with quail immunoreactivity in the dermomyotome and predominantly non-BrdU-labelled quail nuclei in the myotome layer. A few BrdU-labelled quail cells are present in the myotome layer (arrows). (D) Schematic representation of the section shown in A-C. The extent of non-BrdU-labelled donor-derived nuclei in the myotome layer are indicated (bracket, A). Comparable data were obtained in three out of three treated embryos. DML, dorsomedial lip; NT; neural tube.

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