Fig. 1. Effects of unc-86-null mutations on the expression of tph-1::gfp. (A) The position of serotonergic neurons in the head and the axon from HSN (shown in red). The drawing is adapted from Starich et al. (Starich et al., 1995) and White et al. (White et al., 1986). (B) GFP expression of the integrated tph-1-ABCDE::gfp fusion gene in wild-type and unc-86-null adult hermaphrodites. Anterior is towards the left. In wild type, GFP was predominantly expressed in ADF, NSM and HSN. In unc-86-null mutants, GFP expression was observed in ADF, but not in NSM and HSN at any developmental stage. The UNC-86 protein is expressed in NSM and HSN, but not in ADF (Finney and Ruvkun, 1990). Two unc-86-null alleles, n846 and e1416 exhibited the same expression pattern; unc-86(n846) animals are shown. Note that the GFP fusion to the tph-1 sequence has punctate expression pattern, as it appears as two puncta in the ADF neuron.

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