Fig. 3. Expression of DYAD in male and female meiocytes. RNA in situ hybridisation of DYAD antisense RNA to sections of flower buds. Expression is seen (A) in pollen mother cells and (B) in megaspore mother cells at an early stage of ovule development at the time of integument initiation. (C) Magnification of the distal portion of the upper two ovules shown in B. The signal appears localised towards the apical end of the MMC in both ovules. (D) Whole- mount optical section of a cleared ovule at the same developmental stage as in B,C. Bar: 100 µm (A,B) and 20 µm (C,D).

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