Fig. 5. Expression of pAtDMC1::GUS in wild-type and dyad plants. (A,B) Wild type. (C-E) dyad. (A,C) GUS expression is specific to the MMC in stage 2-4 ovules. (B) After meiosis expression is seen in degenerating spores but not in the developing embryo sac at ovule stage 3-2. (D) Pistil containing ovules from stages 2-5 onwards. Ovules towards the basal end of the pistil (right) are slightly more advanced in development than those at the stigmatic end (left). Several of the basal ovules show intense GUS expression in the division products of the MMC consistent with defective meiotic progression. (E) Four examples of division products of the MMC where persistent expression is seen in one or both of the cells. Bar: 20 µm (A-C); 50 µm (D); 10 µm (E).

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