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Fig. 2. Quantitative analysis of thalamic axon growth and branching on membranes from cortical layer 4 (black bars) and cortical layer 5 (white bars). Histograms depict the number of fibers per explant (A), axon length (B) and branching density (C) on each type of membrane substrate. (D) Stripe assay with thalamic axons growing perpendicular to membrane stripes (see Fig. 3). The histogram depicts the branching density observed in each set of stripes. n, number of explants analyzed (A); n, number of fibers examined (B-D). Error bars represent the s.e.m.