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Fig. 6. Quantitative analysis of thalamic axon growth on membranes from NIH3T3-ephrin-A5 cells (black bars) or from control cells (white bars). (A) Number of axons extending from thalamic explants, (B) analysis of axonal length, (C) branch formation under control condition and (D) branch formation after treatment of membranes with PI-PLC enzyme. n, number of explants scored (A); n, number of fibers examined (B-D). ‘n.s.’ indicates not significant and error bars represent the s.e.m.





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