Fig. 7. Immunostaining reveals cellular differentiation within the ectopic neural tubes. Immunostaining for tubulin ß3 (A) and MAP2 (B) confirms the neural identity of the ectopic tubes in E12.5 transgenic embryos. (C,D) Differentiating motoneurons and dorsal root ganglia are identified by Islet-1 immunostaining in wild-type (C) and transgenic (D) neural tubes at E12.5. Ectopic neural tubes are indicated by arrowheads and dorsal root ganglia by arrows. Immunoperoxidase labeling was used in all sections. The section in A was counterstained with Methyl Green. Bar: 50 µm.

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