Fig. 4. The C-terminal part of Su(var)3-7 recruits the endogenous protein and suppresses PEV. (A) Double staining of polytene chromosomes from larvae expressing the HA:7-Ct protein with an anti-HA antibody (red) and anti-Su(var)3-7 antibody (green). The anti-Su(var)3-7 antibody was raised against a N-terminal fragment of the protein, and permits the detection of the endogenous protein but not of the HA:7-Ct protein. hs, heat shocked (B) Effect of different tagged constructs on Heidi variegation. Corresponding eye pigment measurements normalised to the control Heidi/+: Heidi/+ = 100±5.3; Heidi/+; HA:7-B/+ = 881±103; Heidi/+; HA:B-Ct/+ = 88±4.4.

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