Fig. 8. EGFR is co-immunoprecipitated with DE-cadherin and Armadillo in the Drosophila embryo. (A-C) Co-immunoprecipitation of CCC-associated proteins using anti-DE-cadherin (DE-cad) antibody (A), anti-Armadillo (Arm) antibody (B), and anti-EGFR (C). (Left column) Blots were probed with anti-Armadillo antibody. (Center) Blots were probed with anti-EGFR antibody. (Right) Blots were probed with anti-DE-cadherin antibody. Owing to the different sources of the antibodies, it was not possible to normalize the amount of antibody used in conducting the CoIP experiments. Thus, the quantity of proteins pulled down using one antibody differs from that obtained with another antibody. (D) Western blot probed with anti-neurotactin (BP106), documenting the absence of neurotactin in anti-DE-cad and anti-Arm IP products. Western blot contains anti-Arm and Anti-DE-cad IP products (left two lanes) and embryo lysate (right two lanes; A: lysate used for anti-Arm IP; B: lysate used for anti-DE-cad IP). In the lanes loaded with embryo lysate, a band labeled by anti-neurotactin antibody proves that neurotactin is present in the lysate.

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