Fig. 2. L mediates N signaling in the ventral eye. (A) Wild-type, third instar disc double-stained with anti-Elav (red) and anti-Dlg (green). (B) UAS-N^intra/dpp-Gal4 caused overgrowth in dorsal and ventral domains (arrows). (C) L^si mutant showed a loss of the ventral domain. (D) Loss of L suppressed UAS-N^intra/dpp-Gal4 phenotype in the ventral domain. The putative ventral domain is outlined. Dorsal overgrowth persisted, such that the tissue had a crumpled, uneven appearance. (E) UAS-N^DN/ey-GAL4 caused a general loss of tissue. (F) Co-expression of UAS-L and UAS-N^DN specifically restored the ventral eye. (G) L^si/+ adult eye showed a characteristic anterior notch (yellow arrow). (H) Removing a copy of N in L^si/+ animals increased the severity of the L eye phenotype.

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