Fig. 5. L is a novel protein with preferential expression anterior to the furrow. (A) Overlapping cosmids spanning the 51A2-B1 regions were isolated. Cos D3, but not cos F7, rescued the L phenotype and the lethality of L^P17 allele, which has a P-element inserted in the first exon. E: EcoRI site. (B) The putative L protein contains a poly-glutamine rich region (underlined). Arrowhead indicates the P-element insertion site of L^P17. (C) Sequences of the C terminus of L and its related proteins over a span of 67 amino acids: bee (BI509118) (43% identical; 63% positive), mouse (AK003638) and human (BC007416) (37% identical; 53% positive). In the last 30 amino acids, 56% are identical. The red letters indicate identical amino acids and green ones indicate similar amino acids. (D) Full-length L cDNA detected a single band on a northern blot of total RNA extracted from larvae of all stages. (E) Embryo of L^rev6-3/L^rev6-3 (arrowhead), a loss-of-function allele, has no detectable level of L protein (red). In L^rev6-3/+ embryo (arrow) the protein was readily detected. The genotype of the embryos was distinguished with green fluorescent protein marker (not shown). (F,G) In third instar eye/antenna discs, L transcripts, detected by RNA in situ hybridization (F), and L protein, detected by antibody (G), are most intense in the outer antenna ring and in the eye disc anterior to the furrow

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