Fig. 5. Depletion of XTcf3 does not rescue organizer formation in VegT^– embryos. (A) Phenotypes of uninjected controls, XTcf3^– (blue; 3 ng T1), VegT^– (red; 6 ng) and XTcf3^–/VegT^– (mauve; 3 ng T1+6 ng VegT oligo) at the tailbud stage. XTcf3^–/VegT^– embryos resemble the VegT^– embryo. (B) Real-time RT-PCR analysis of XTcf3^–/VegT^– embryos. Sibling embryos of those shown in A were collected at the indicated stages during gastrulation and analyzed for expression of dorsal/ventral patterning markers. siamois and Xnr3 are up-regulated in XTcf3^– embryos and remain at high levels in XTcf3^–/VegT^– embryos. Goosecoid and Xnr6 are up-regulated in XTcf3^– embryos but are not expressed in either VegT ^– or XTcf3^–/VegT^– embryos. Chordin, Xnr1 and Xsox17 have a similar pattern of expression, but are not as severely affected as goosecoid and Xnr6. Bmp4 is unaffected by depletion of either XTcf3 or VegT.

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