Fig. 1. The regulation of zygotic transcription by both the VegT and the Wnt pathway. Wild-type, VegT^–, ß-catenin^– and VegT^–/ß-catenin^– embryos were frozen at 2-hourly intervals from the mid-blastula (stage 8) to the late gastrula (stage 11.5) stages and analysed by real-time RT-PCR. The results are displayed as histograms to show the relative levels of expression of molecular markers representing (A) genes that require both pathways for the initiation of their expression, (B) direct targets of the Wnt pathway, (C) genes that require VegT but not ß-catenin for the initiation of their expression, (D) genes activated normally in both VegT^– and ß-catenin^– embryos. In each case, ornithine decarboxylase (ODC) is used as a loading control (data not shown), and each bar is normalized to the level of ODC expression.

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