Fig. 7. Organizer gene expression is rescued at the early neurula stage by BMP blockade in ß-catenin^– but not VegT^– embryos. Wild-type (uninjected, U), VegT^– thioate (V), m ß-catenin^– thioate (T) and m/z ß-catenin^– morpholino (M) embryos, and siblings injected with cm-BMP7 (cmB) at the 8-cell stage (600 pg; 1 ventral vegetal cell) were frozen at the early gastrula (stage 10.5) and early neurula stage (stage 13) and analysed by real-time RT-PCR for organizer gene expression. The results are displayed as histograms to show the relative levels of expression. In each case, ornithine decarboxylase (ODC) is used as a loading control (data not shown), and each bar is normalized to the level of ODC expression.

Return to article