Fig. 3. Six3 is involved in proximodistal patterning of the eye. Dorsal views, anterior is towards the left; developmental stages, morpholinos injected and molecular markers used for in situ hybridization are indicated; all Six3 morphants used for analysis exhibit a mild (small eye) phenotype. (B,F,J,N) Broken yellow line outlines optic vesicles. (A,B) Six3 reduction results in loss of Vax1 expression in the optic stalk. (C,D) Loss of forebrain structures and fusion of distal retina structures is visualized by double in situ hybridization with Rx2 (red) and Emx1 (purple). Bar indicates region of remaining Emx1-expressing tissue. (E,F) Retinal Rx3 expression is lost in Six3 morphants. (G-N) In Six3 morphants Six3 is lost and Pax6 is reduced in the retina at six-somite stage (21). mb, midbrain.

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