Fig. 4. Cooperation of Pax6 and Six3 in eye development. (A-D) Dorsal views of embryos at organogenesis stage, anterior is towards the left; injected morpholinos are indicated. (B) MoPax6 results in development of small eyes compared with control embryos (A). (C,D) Co-interference with Six3 and Pax6 function results in formation of either small (mild) or cyclopic eyes (intermediate). Arrow in D indicates proximal retinal structures lost; arrowhead indicates forebrain lost. (E) MoPax6/MoSix3 co-injection (concentrations in mM) (n=473) shifts the mild (blue) towards the intermediate phenotype (red) when compared with MoPax6/MoGFP (n=50) or MoSix3/MoGFP-injected embryos (n=50). ey, eye; hb, hindbrain; ot, optic tectum.

Return to article