Fig. 7. Xpygo depletion results in a ventralized phenotype. (A) Maternal Xenopus pygo (Xpygo) mRNA transcripts are depleted in a dose-dependent manner upon injection of an antisense Xpygo oligo into oocytes. mRNA levels were assayed 24 hours after oligo injection. (B) Uninjected control embryo (above; blue) at stage 36, and embryos derived from sibling oocytes injected with antisense Xpygo (below; mauve). Xpygo^– embryos are ventralized. (C) Relative expression of markers normalized to ODC in wild type and Xpygo^– embryos at late blastula (9.5) and gastrula (10.5) stages. Expression of dorsal markers is reduced in Xpygo^– embryos.

Return to article