Fig. 2. Loss of PTEN function rescues InR null mutations. (A) Pten^2L117/Pten^2L100 mutant larvae have increased PtdInsP₃ levels. Data are presented as PtdInsP₃:PtdInsP₂ ratios and are standardized to the wild-type y w control. The experiment was performed twice with quantitatively similar results. (B) The Pten^2L117/Pten^2L100 mutant larva (top) is increased in size compared to the y w control. (C) Scanning electromicrograph (SEM) of a dorsal view of a y w control fly. Bar (above C) is 200 µm in all cases. (D) Loss of PTEN function (Pten^2L117/Pten^2L117) selectively in the head using the eyFlp system (Newsome et al., 2000) results in the overproliferation of the head. (E) Loss of InR function (InR³²⁷/InR³²⁷) selectively in the head using the eyFlp system results in a pinhead. (F) Concomitant loss of InR and PTEN function (Pten^2L117/Pten^2L117; InR³²⁷/InR³²⁷) in the head is sufficient to suppress the growth and proliferation defect associated with InR single mutants. (G) The 2nd instar lethality associated with the InR³⁰⁴/InR³²⁷ allelic combination is rescued to the pharate adult stage in the Pten^2L117/Pten^2L100; InR³⁰⁴/InR³²⁷ double mutant combination (left). (Right) A y w control. (H) The frequency of rescue to the pupal stage of InR³⁰⁴/InR³²⁷ (InR1) (n=15 crosses) and InR³⁰⁴/InR²⁵ (InR2) (n=5 crosses) by the partial loss-of-function Pten^2L117/Pten^2L100 combination. ‘Expected’ denotes the expected Mendelian frequency for complete rescue to the pupal stage for the genotype: y w; Pten^2L117/Pten^2L100; InR³⁰⁴/InR³²⁷ from the cross (y w; Pten^2L117; InR³⁰⁴/Cyo^TM6B x y w; Pten^2L100; InR³²⁷/Cyo^TM6B).

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