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Fig. 4. Pten regulates size, metabolism and survival in an opposite manner to positive components of the insulin pathway like Chico. (A) A Pten2L117/Pten2L100 mutant fly (right) compared to the y w control (left). (B) Pten2L117/Pten2L100 mutant flies weigh more than the y w control (data not shown) or to Pten mutants rescued with the Pten genomic rescue construct (n=10). (C) Pten2L117/Pten2L100 mutant flies have decreased glycogen and lipids compared to the y w control (data not shown) or to Pten mutants rescued with the Pten genomic rescue construct (n=10). The glycogen data are transformed by one log10 power. (D) Pten2L117/Pten2L100 mutant flies are hypersensitive to water-only starvation conditions compared to a starvation resistant chico1/chico1 mutant. All assays were performed at least 2 times independently.





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