Fig. 1. fu mutations generate ovarioles with egg chambers containing abnormal numbers of germ cells. All ovarioles are oriented with anterior towards the top or right. (A-F) DAPI staining of wild-type (A), fu^JB3 (B-D,F) and fu¹ (E) ovarioles. Arrowheads indicate compound egg chambers (B,C,E,F) or chambers with less than 16 germline cells (D). (B) Arrow indicates a degenerating egg chamber. (C) Arrow indicates apposed egg chambers. (F) Arrows indicate two vitellogenic oocytes in the same egg chamber. (G-H) Wild-type (G) and fu^JB3 (H) egg chambers stained with DAPI (G,H), anti-Orb antibodies (G',H') and phalloidin (G'',H''). Arrowheads indicate Orb-expressing oocytes. Scale bars: 10 µm.

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