Fig. 2. fu is required for correct encapsulation of germline cysts by prefollicular cells in the germarium. All germaria are oriented with the anterior towards the top or top right-hand corner. (A-B'') Double labeling of wild-type (A-A'') and fu^JB3 (B-B'') germaria with anti-Fas III (A,B) and anti--Spectrin (A',B') antibodies, both of which stain prefollicular cells and their descendants (anti--Spectrin also stains fusomes, the branched cytoplasmic structures that link all the cystocytes of a developing cyst). FIII denotes Fas III. Spec denotes -Spectrin. Basal membranes are defined by contacts with the peripheral basal lamina. Arrowheads in A indicate prefollicular cells extending cell processes centripetally and the arrow indicates prefollicular cells that have begun the intercalation process (germarial region 2b/3). (C,D) fu^JB3 germaria stained with anti-Fas III antibodies. (C',D') Corresponding DAPI nuclear staining. Arrows in C,D indicate irregular intercalation between prefollicular cells. Arrowheads in C,D indicate somatic cells, within egg chambers, weakly staining for Fas III. Asterisks in C,C',D,D' highlight peripheral aggregates of somatic cells. (D') In the focal plane shown here, 15 nurse cell nuclei are visible in the budding egg chamber, whereas additional nurse cell nuclei are visible in other focal planes of this chamber (data not shown). Scale bars: 10 µm.

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