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Fig. 3. In situ hybridization of Hegfl mRNA in hypophysectomized (hyx) progesterone (P4)-treated pregnant (days 4 and 5) hamster uterus. Dark-field photomicrographs of representative uterine sections are shown. Pregnant hamsters were hypophysectomized on day 2 (0900 hours) and treated with P4 (1 mg/hamster) daily from day 2. Whole uterine tissues were collected on the morning of day 4 (0900 hours). Implantation sites were collected on the afternoon of day 4 (1600 hours) and morning of day 5 (0900 hours) after an intravenous injection of blue dye as described in the Materials and Methods. Day 5 implantation sites obtained from sham operated pregnant hamsters treated with oil, were used as control. am, antimesometrial side; bl, blastocyst; le, luminal epithelium; m, mesometrial side; myo, myometrium; s, stroma.





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